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C3766
Sigma
Cholesterol Esterase from bovine pancreas
lyophilized powder, ≥200 units/g protein
| Synonym: | Sterol-ester acylhydrolase |
| CAS Number: | 9026-00-0 |
| Enzyme Commission (EC) Number: | 3.1.1.13 ( BRENDA | IUBMB ) |
| EC Number: | 232-808-6 |
| MDL number: | MFCD00071052 |
Description
| Analysis Note | Protein determined by biuret. |
| Other Notes | Partially purified |
| Unit Definition | One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37 °C in the presence of taurocholate. |
| Application | Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol. Optimum pH range: 6-8 Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine |
Properties
| form | lyophilized powder |
| composition | protein, 30-65% |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
